Use of Random Primer Extension for Concurrent Amplification and Nonradioactive Labeling of Nucleic Acids
β Scribed by J. Mackey; M. Darfler; P. Nisson; A. Rashtchian
- Book ID
- 102966392
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 782 KB
- Volume
- 212
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A method has been developed for simultaneous radiolabeling and amplification of DNA hybridization probes. The method is termed cycled primer extension (CPE). CPE is a series of temperature-driven reactions in which template DNA is successively denatured and extended by a thermostable primer-dependen
We have used M13 single-stranded DNA bound by uv to small pieces of nylon membrane for the synthesis of biotinylated single-stranded DNA probes. The labeling method requires a large fragment of DNA polymerase I and random hexanucleotides. There is no need for previous linearization of the template.