## Abstract Ultrafiltration/diafiltration (UFDF) is commonly utilized in the purification of recombinant proteins to concentrate and buffer exchange the product. It is often the final step in the purification process, placing the protein in its final formulation and clearing small molecules introdu
✦ LIBER ✦
Use of protein charge ladders to study electrostatic interactions during protein ultrafiltration
✍ Scribed by Mareia Frost Ebersold; Andrew L. Zydney
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 355 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0006-3592
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## Abstract The adsorption equilibria of bovine serum albumin (BSA), γ‐globulin, and lysozyme to three kinds of Cibacron blue 3GA (CB)‐modified agarose gels, 6% agarose gel‐coated steel heads (6AS), Sepharose CL‐6B, and a home‐made 4% agarose gel (4AB), were studied. We show that ionic strength has