Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections

✍ Scribed by T. F. C. Batten; C. R. Hopkins

• Book ID: 104787928
• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 882 KB
• Volume: 60
• Category: Article
• ISSN: 1432-119X
• DOI: 10.1007/bf00500659

✦ Synopsis

ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cells was identified as containing numerous round or ovoid 170--300 nm secretory granules.