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Use of polynucleotide phosphorylase in sequence determination of oligonucleotides

โœ Scribed by J.T. Madison; R.W. Holley; J.S. Poucher; P.H. Connett


Book ID
115742609
Publisher
Elsevier Science
Year
1967
Weight
184 KB
Volume
145
Category
Article
ISSN
0005-2787

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Isolation of a polynucleotide phosphoryl
โœ Portier, C. ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› Springer ๐ŸŒ English โš– 576 KB

Insertion in an episome of a kanamycine-resistant element (Tn5) at the polynucleotide phosphorylase gene level, results, after transduction into a wild strain, by the loss of activities specific to polynucleotide phosphorylase. A low phosphorolytic activity is nevertheless detectable in crude extrac