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Use of polymerase chain reaction catalyzed by Taq DNA polymerase for site-specific mutagenesis

✍ Scribed by Kadowaki, Hiroko; Kadowaki, Takashi; Wondisford, Fredric E.; Taylor, Simeon I.

Book ID
122015934
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
562 KB
Volume
76
Category
Article
ISSN
0378-1119

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We investigated the effect of primer-template mismatch on the efficiency of polymerase chain reaction. For primers with T, C, or G as the 3 nucleotide, Thermus aquaticus (Taq) DNA polymerase was highly specific for template complementarity to this base, but was somewhat less constrained opposite the