Use of multi-staining flow cytometry to characterise the physiological state of Escherichia coli W3110 in high cell density fed-batch cultures

Studies related to the scale-up of high-

✍ Christopher J. Hewitt; Gerhard Nebe-Von Caron; Britta Axelsson; Caroline M. McFa 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 650 KB

Multiparameter flow cytometric techniques developed in our laboratories have been used for the "atline" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m 3 ) and bench (5 × 10 -3 m 3 ). In addition, at the bench scale, experiments were undertak