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Use of monoclonal anti-idiotypic antibody to P3-X63Ag8 myeloma protein for analysis and purification of B lymphocyte hybridoma products

✍ Scribed by Jan Gheuens; Dale E. McFarlin


Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
326 KB
Volume
12
Category
Article
ISSN
0014-2980

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✦ Synopsis


Abstract

A lymphocyte hybridoma, termed 80 V5B4, that secretes a syngeneic anti‐idiotypic antibody to P3‐×63Ag8 (×63) myeloma protein, is described. A sensitive radioimmunoassay to detect ×63 myeloma protein was developed with this monoclonal anti‐idiotypic antibody which can also be used to remove the ×63 myeloma protein from hybridoma products.