Use of fructose-2,6-diphosphate to assay
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Louis H. Schliselfeld; Moris J. Danon
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Article
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1996
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Elsevier Science
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English
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Objective: Use fructose-2,6-diphosphate (fru-2,6-P2) for measuring phosphofructokinase (PFK) activity in muscles. Design and Methods: PFK activity was measured at 2 mmol/L MgCI2 and 5 mmol/L adenosine triphosphate (ATP) (mol/L MgCI2:mol/L ATP 0.4) without and with fru-2,6-P 2. Results: Human muscle