Use of methyl and ethyl acetate as organic modifiers in reversed-phase high-performance liquid chromatography: Application to impurity control in bulk drug steroids

Prediction of experimental parameters in

Prediction of experimental parameters in combined isocratic and gradient elution reversed phase high performance liquid chromatography (RP-HPLC) using acetonitrile as organic modifier

✍ Molnar, I. 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 277 KB

## Abstract The use of gradient elution in RP‐HPLC is increasing. Aqueous buffers, such as phosphate at pH 1.7, with acetonitrile as the organic modifier are particularly advantageous as eluents with linear elution strength (LES). In the isocratic mode, under the experimental conditions used, fairl