Use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge
✍ Scribed by Kensuke Ochi; Gouping Chen; Takashi Ushida; Satoshi Gojo; Kaoru Segawa; Hitoshi Tai; Kenju Ueno; Hiroyuki Ohkawa; Taisuke Mori; Akira Yamaguchi; Yoshiaki Toyama; Jun-ichi Hata; Akihiro Umezawa
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 596 KB
- Volume
- 194
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
Controlled regeneration of bone or cartilage has recently begun to facilitate a host of novel clinical treatments. An osteoblast line, which we isolated is able to form new bone matrix in vivo within 2 days and exhibits a mature osteoblast phenotype both in vitro and in vivo. Using these cells, we show that cuboidal bones can be generated into a predesigned shaped‐bone with high‐density bone trabeculae when used in combination with a modified poly‐DL‐lactic‐co‐glycolic acid (PLGA)‐collagen sponge. PLGA coated with collagen gel serves as a good scaffold for osteoblasts. These results indicate that mature osteoblasts, in combination with a scaffold such as PLGA‐collagen sponge, show promise for use in a custom‐shaped bone regeneration tool for both basic research into osteogenesis and for development of therapeutic applications. © 2002 Wiley‐Liss, Inc.