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Use of glucomannan for the separation of DNA fragments by capillary electrophoresis

โœ Scribed by Tomonori Izumi; Masato Yamaguchi; Kenichi Yoneda; Toshiaki Isobe; Tsuneo Okuyama; Tomotaka Shinoda

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
528 KB
Volume
652
Category
Article
ISSN
1873-3778

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## Abstract Polyacrylamide gels are unstable in 50โ€ฮผm internal diameter capillaries at electric fields greater than about 500 V cm^โˆ’1^ due to the formation of bubbles, which destroy the separation. We describe the use of a modified polyacrylamide capillary gel that is stable at electric fields of a