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Use of gene fusions of the structural gene sdaA to purify L-serine deaminase 1 from Escherichia coli K-12

โœ Scribed by Hongsheng SU; John MONIAKIS; E. B. NEWMAN


Book ID
115129826
Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
850 KB
Volume
211
Category
Article
ISSN
1432-1327

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Fusion of the lac genes to the promotor
โœ Josephsen, Jytte ;Hammer-Jespersen, Karin ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 517 KB

Phage Mu has been inserted into the structural gene for cytidine deaminase (cdd). By the use of phage 2 (lac, Mu) the promoter for the cdd gene has been fused to lacZ. In these strains lacZ expression is regulated by the cytR repressor protein and is therefore induced by cytidine. The fusion strains