Use of four-colour flow cytometry to evaluate conjugate formation between human peripheral blood mononuclear cells and tumour target cells
✍ Scribed by Malcolm A. King; Monica A. Radicchi-Mastroianni
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 575 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0196-4763
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✦ Synopsis
A four-colour flow cytometry technique is described for the determination of the number and phenotype of conjugate-forming mononuclear effector cells from human blood. The discriminatory power of previously described techniques was improved by labelling the effector cells with antibodies that simultaneously idenaed natural killer (NK), T, and myeloid cells and by labelling the tumour target cells with fluorescein octadecyl ester (FOE), so that cell conjugates could be differentiated from nonbinding effector cells more effectively than by the use of light scatter. The simultaneous characterisation of the three classes of conjugate-form3ng cells within a heterogeneous human peripheral blood mononuclear cell (PBMC) population made it possible to investigate conjugate formation by each subpopulation without purification, or semipurification (e.g., by monocyte re-Natural killer (NK) cells are key mediators of natural immunity, playing a vital role in immunosurveillance against cancer and in first-line defense against infectious agents (23). They are the major lymphocyte popula-