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Use of formamide in nucleic acid reassociation

✍ Scribed by Schmeckpeper, B. J.; Smith, Kirby D.

Book ID
126294753
Publisher
American Chemical Society
Year
1972
Tongue
English
Weight
892 KB
Volume
11
Category
Article
ISSN
0006-2960

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✍ Chanchal Sadhu; Santanu Datta; K.P. Gopinathan πŸ“‚ Article πŸ“… 1984 πŸ› Elsevier Science 🌐 English βš– 403 KB

The optimal parameters in the use of nuclease S1 in DNA reassociation kinetics in the presence of formamide have been determined. The conditions are especially suitable for the study of DNA rich in mole percent GC. A 10-fold dilution of the reassociation samples leading to a decrease in both NaCl an