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Use of fluorescent substrate 4-mup in the detection of biotin-labeled DNA probes

✍ Scribed by Kan-yan, Xiao ;Zhi-heng, Liu ;Shen, Qu ;Shan-shu, He ;Yao-zu, Deng

Book ID
112927684
Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
381 KB
Volume
13
Category
Article
ISSN
0257-716X

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## Abstract A biotin‐labeled DNA probe specific for the im‐ mediate early gene of the cytomegalovirus (CMV) strain AD 169 was generated with the polymerase chain reaction. Nucleic acid hybridisation was carried out with CMV‐infected T‐lymphoblastoid cells (MOLT4) after 4 hr to 6 days of culture. Bi