A rapid and sensitive HPLC-based method for quantitating isoaspartate levels in peptides and proteins is described. The analyte is incubated for 40 min with S-adenosyl-L-methionine and the commercially available enzyme protein L-isoaspartyl methyltransferase. Methylation of isoaspartyl sites results
โฆ LIBER โฆ
Use of fluorescamine in the chromatographic analysis of peptides from proteins
โ Scribed by N. Nakai; C.Y. Lai; B.L. Horecker
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 410 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0003-2697
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## Synopsis A vibrational force field for the polypeptide chain has been developed for normal-mode analysis of such molecules. It can reproduce observed frequencies of known structures to within about 5 cm-l. We review the application of this technique to conformational problems in peptides (@-tur