Use of Ferric Chloride-Methanol Reagent for the Analysis of Fatty Acid Composition of Lipids
β Scribed by Dasgupta, F. ;Kartha, K. P. R. ;Pitke, P. M. ;Singh, P. P.
- Publisher
- John Wiley and Sons
- Year
- 1981
- Weight
- 313 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0931-5985
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β¦ Synopsis
Abstract
A solution of anhydrous ferric chloride in methanol (15% w/v) converts fatty acids into their methyl esters within 10 minutes under reflux. This reagent is comparable to methanolic hydrogen chloride in its performance and can be applied for both transesterification of lipids as well as for the esterification of free fatty acids.
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The use of electrospray Ionization (ESI) tandem mass spectrometry (MS/MS) for the structural determination of the lipid A components of the psychrophilic bacterium Pseudoalteromonas haloplanktis TAC 125 is reported. The lipid A contains the classical bisphosphorylated beta-(1' --> 6)-linked D-glucos