Use of enzyme-labelled protein G assay for the detection of antiBorrelia burgdorferiantibodies in wild animal sera
✍ Scribed by Dominique Deruaz; Pierre Eid; Jean Deruaz; Antoine Sempéré; Catherine Bourgouin; François Rodhain; Claudine Pérez-Eid
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 471 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0393-2990
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✦ Synopsis
A modified ELISA was developed for the detection of anti-Borrelia burgdorferi (Bb) IgG antibodies in wild animal sera based on an Enzyme-Labelled-protein G Assay (ELGA). Microplates were coated with an extract of Bb sensu stricto strain (SV1) as antigen. Specific antibodies of the serum samples were detected by a peroxidase-labelledprotein G. Using comparative immunodiagnosis by means of a passive hemagglutination test (HA), ELGA was tested on 82 roe-deer blood samples. A correlation was found between the two methods (r = 0,66). Good reproducibility of titers was observed by ELGA technique. A minimal crossreactivity was discovered with Leptospira. ELGA could facilitate the recognition of specific antibodies in collections of wild animal sera.