Use of enzyme label for quantitative evaluation of liposome adhesion on cell surface: Studies with J774 macrophage monolayers
✍ Scribed by Vladimir S. Trubetskoy; Elena V. Dormeneva; Vladimir P. Tsibulsky; Vadim S. Repin; Vladimir P. Torchilin
- Book ID
- 102630253
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 447 KB
- Volume
- 172
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method for quantitation of cell surface-bound liposomes utilizing J774 macrophage monolayers is developed. Surface-bound biotinyl-containing and 125I-labeled liposomes were quantified with avidin-peroxidase in an ELISA-like assay. Peroxidase substrate absorbance values were recalculated into the absolute amount of liposomal lipid using a special calibration plot. Total liposome uptake by macrophages was determined following the binding of 125I radioactivity. The approach suggested allows quantitative evaluation of the changes in the content of surface-adhered liposomes during their interaction with cells in vitro.