Use of electroporation for high-molecular-weight DNA-mediated gene transfer
โ Scribed by Margaret M. Jastreboff; Etsuro Ito; Joseph R. Bertino; Ramaswamy Narayanan
- Book ID
- 115892163
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 808 KB
- Volume
- 171
- Category
- Article
- ISSN
- 0014-4827
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We investigated gene transfer in finfish and shellfish via electroporated sperm. The mobility of sperm, the fertilization rate, the hatching rate, gene transfer rate, and abnormality rate of derived embryos were primarily dependent on the voltage level and concentration of DNA during electroporation
Previous attempts to extract high molecular weight DNA from hermatypic corals have failed because the starting material was mature tissue. The following paper describes the isolation of DNA from coral sperm which is of high molecular weight, and is suitable for DNA hybridization, digestion with rest