Application of Isotopic Ratio Mass Spect
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Florence Grand; Izzet Kilinc; Albert Sarkis; JΓ©rΓ΄me Guitton
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Article
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2002
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Elsevier Science
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English
β 86 KB
The reaction of demethylation mediated by cytochrome P450 (CYP) leads to the equimolar production of demethylated metabolite and formaldehyde. From a 13C-substrate labeled on a carbon of the methyl moiety, [13C]formaldehyde (H13CHO) is liberated. A highly sensitive and specific assay involving the o