Use of d-glucosaminic acid as an internal standard in single-column accelerated amino acid analysis of physiological fluids
✍ Scribed by Collette Stacey-Schmidt; Peter Berg; Morey W. Haymond
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 303 KB
- Volume
- 123
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
D-Glucosaminic acid (DGA) was investigated as an early-eluting internal standard under single-column cation-exchange chromatography conditions because it elutes early in the analysis between urea and aspartic acid and obeys Beer's law. The precision and accuracy of data obtained with this internal standard were indistinguishable from those obtained with the conventionally used, late-eluting internal standard S-@(4-pyridylethyl)-L-cysteine (4-pet). DGA was stable when stored over 6 months at -20 or -70°C but was not stable to acid hydrolysis. The use of D-glucosaminic acid as an internal standard provides considerable flexibility in the development of analytic methodologies for rapidly analyzing selected amino acids in physiological fluids.
' To whom requests for reprints should be addressed. Reagents. Citric acid monohydrate (Fisher * Abbreviations used: 4-pet, S-@(4-pyridylethyl)-L-Scientific), anhydrous lithium hydroxide cysteine; DGA, D-glucosaminic acid.
(Fisher Scientific), thiodiglycol (Sigma),