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Use of confocal microscopes in conoscopy and ellipsometry 1 Electromagnetic theory

โœ Scribed by Varga, Peter

Book ID
115349072
Publisher
The Optical Society
Year
2000
Tongue
English
Weight
95 KB
Volume
39
Category
Article
ISSN
1559-128X

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## Abstract We describe a method to obtain the brightness and number of molecules at each pixel of an image stack obtained with a laser scanning microscope. The method is based on intensity fluctuations due to the diffusion of molecules in a pixel. For a detector operating in the analog mode, the v