Use of complexing agents for selective separation in high-performance capillary electrophoresis: Chiral resolution via cyclodextrins incorporated within polyacrylamide gel columns
✍ Scribed by A. Guttman; A. Paulus; A.S. Cohen; N. Grinberg; B.L. Karger
- Book ID
- 104145760
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 760 KB
- Volume
- 448
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
The incorporation of a complexing agent within a polyacrylamide gel column provides a general means of manipulating the selectivity of a capillary electrophoresis separation. As an example of this approach, chiral resolution of dansylated amino acids by high-performance capillary electrophoresis has been achieved by adding fi-cyclodextrin within the gel matrix. A retention model has been developed which can be used for selectivity optimization. Various parameters, such as concentration of cyclodextrin, addition of methanol to the buffer, and temperature, have been examined in terms of their influence on retention and selectivity. From' this study highperformance separations have been developed. Efficiencies as high as 100 000 plates in 15 cm have been achieved.