A method which uses 1-naphthylisocyanate as an HPLC precolumn derivatization reagent for amino acid analysis is described. Derivatization is carried out by adding the isocyanate dissolved in dry acetone to a buffered amino acid solution followed by extraction of the excess reagent with cyclohexane.
✦ LIBER ✦
Use of chromogenic and fluorescent oxycarbonyl chlorides as reagents for amino acid analysis by high-performance liquid chromatography
✍ Scribed by H. Bru¨ckner; M. Lu¨pke
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 924 KB
- Volume
- 697
- Category
- Article
- ISSN
- 1873-3778
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Figure 1. Reaction of D/L-amino acids with R(À)-DBD-PyNCS.