Use of Chemically Modified Silica with β-Diketoamine Groups for Separation of α-Lactoalbumin from Bovine Milk Whey by Affinity Chromatography
✍ Scribed by Alessandra Gambero; Lauro T. Kubota; Yoshitaka Gushikem; Cláudio Airoldi; José M. Granjeiro; Eulázio M. Taga; Edésio F.C. Alcântara
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 105 KB
- Volume
- 185
- Category
- Article
- ISSN
- 0021-9797
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✦ Synopsis
area. Generally, organic matrices like agarose, cellulose, and Silica gel surface was chemically modified with b-diketoamine composite polymers have been used (4).
groups by reacting the silanol from the silica surface with 3-Recent studies demonstrated that some mineral solids may aminopropyl-triethoxysilane and 3-bromopentanedione. With this be used as adsorbents of proteins. The surfaces of these material, copper ions were adsorbed from aqueous solutions. The materials are highly developed and permit the immobilizachemical analysis of the silica-gel-immobilized acetylacetone protion of functional groups. Among these solids, silica gel may vided a quantity of 0.67 mmol g 01 of organic groups attached to be used to this aim. The silica gel surface is covered with the support and 0.63 mmol g 01 of copper. This material was used silanol groups (Si-OH), which determine its chemical beas a stationary phase in IMAC (immobilized metal affinity chrohavior (5-11). matography), to separate a-lactoalbumin from bovine milk whey.
The results showed an efficient separation in the chromatographic
The use of silica gel as a matrix for the immobilization column. The possibility of reutilization of the stationary phase was of molecules with organofunctional groups has some advanalso investigated. ᭧ 1997 Academic Press tages over organic matrices, such as high surface area, a