✦ LIBER ✦
Use of avidin-sepharose to lsolate and identify biotin polypeptides from crude extracts
✍ Scribed by Judith A. Swack; Gary L. Zander; Merton F. Utter
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 833 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A method of isolating and identifying biotin polypeptides from crude cellular extracts is described. Protein samples are run on small avidin-Sepharose columns. After washing away nonspecifically bound protein, the biotin enzymes are eluted using an SDS-urea solution. The inactive polypeptides are then electrophoresed on SDS-polyacrylamide gels. The biotin polypeptides in the gels are identified by using fluorescent avidin or by analyzing for radioactive biotin-labeled polypeptides. A sensitive method for assaying biotin using avidin-Sepharose is also described.