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Use of an RNA folding algorithm to choose regions for amplification by the polymerase chain reaction

✍ Scribed by Luke Pallansch; Howard Beswick; John Talian; Peggy Zelenka

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 634 KB
Volume: 185
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(90)90254-7

No coin nor oath required. For personal study only.

✦ Synopsis

We have used the program FOLD, which employs the Zuker folding algorithm, to identify regions of stable secondary structure in three chicken proto-oncogene mRNAs: c-src, c-myc, and c-fos. We have found that use of reverse transcriptase to synthesize a cDNA template for amplification by the polymerase chain reaction is successful only if the region chosen for amplification does not contain stem structures with calculated free energies less than -14 kcal/mol.

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