Use of Adsorptive Transfer Stripping Voltammetry for Analyzing Variations of Cytosine Methylation in DNA

✍ Scribed by Andrea Ioannou; Despina Alexiadou; Sofia Kouidou; Stella Girousi; Anastasios Voulgaropoulos

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 325 KB
• Volume: 21
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200900274

✦ Synopsis

The electrochemical behavior and thermal stability of double stranded oligonucleotides containing 5-methyl-cytosine and 7-deaza-guanosine as nucleotide analogues, as well as of Jurkat genomic DNAs methylated to different degree were studied by ACV and SWV and by thernal denaturation analysis. ACV and SWV combined with thermal denaturation analysis of the natural and modified oligonucleotides gave information regarding the presence of methylation and the concomitant conformational changes. ACV and SWV of Jurkat DNA mixtures methylated to different degrees revealed a decrease of the peak heights with increasing methylation, indicating an increase of structural rigidity, in agreement with the thermal denaturation data. These results verify the, possibly local, conformational changes introduced by DNA methylation. The results obtained in all cases were reproducible.