Use of a stopped-flow/T-format spectrofluorimeter for simultaneous kinetic analyses

✍ Scribed by B. Gala; A. Gómez-Hens; D. Pérez-Bendito

• Book ID: 102982565
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 599 KB
• Volume: 310
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00153-q

✦ Synopsis

The potential of the joint use of a T-format spectrofluorimeter and the stopped-flow mixing technique in simultaneous kinetic determinations was studied. For this purpose, the direct resolution of a mixture of two analytes (neomycin and promethazme), by use of a condensation and an oxidation reaction that yield reaction products with similar maximum excitation wavelengths but different emission wavelengths was chosen. The determination was readily accomplished in a few seconds by simultaneously measuring the respective initial rates in the corresponding emission channels. Mixtures of neomycin and promethazine in ratios between 8:l and 1:9 were thus satisfactorily resolved. The relative standard deviation was l.O-1.8%. The proposed method was applied to the analysis of a commercially available pharmaceutical preparation, with a recovery of 99.3-102.0% for neomycin and 88.8-93.0% for promethazine.