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Use of a mutant strain of the cyanobacterium Synechococcus R2 for the determination of nitrate

✍ Scribed by Francisco Madueño; Miguel G. Guerrero


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
262 KB
Volume
198
Category
Article
ISSN
0003-2697

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✦ Synopsis


A sensitive procedure for the determination of nitrate within the 1- to 50-microM range is described. The method is based on the photoreduction of nitrate to nitrite by whole cells of a nitrite reductase-less mutant strain of the unicellular cyanobacterium Synechococcus R2. Cell suspensions of this cyanobacterium retain high levels of nitrate photoreduction activity for at least 2 months when maintained in the presence of dimethyl sulfoxide at -70 degrees C.


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