Systematic studies of the parameters influencing transformation efficiencies in Synechococcus R2 and its spontaneous pUH24 plasmid-minus variant S60, have led to substantial increases in these frequencies (up to 70 times for R2 and 9 times for S60) compared to those obtained by using previously publ
Use of a mutant strain of the cyanobacterium Synechococcus R2 for the determination of nitrate
✍ Scribed by Francisco Madueño; Miguel G. Guerrero
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 262 KB
- Volume
- 198
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A sensitive procedure for the determination of nitrate within the 1- to 50-microM range is described. The method is based on the photoreduction of nitrate to nitrite by whole cells of a nitrite reductase-less mutant strain of the unicellular cyanobacterium Synechococcus R2. Cell suspensions of this cyanobacterium retain high levels of nitrate photoreduction activity for at least 2 months when maintained in the presence of dimethyl sulfoxide at -70 degrees C.
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