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Use of a microbial model for the determination of drug effects on cell metabolism and energetics: Study of citrulline-malate

✍ Scribed by Joël Briand; Henri Blehaut; Régis Calvayrac; Danielle Laval-Martin

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
994 KB
Volume
13
Category
Article
ISSN
0142-2782

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✦ Synopsis

Euglena gracilis can be used as a microbial model to study the effect of drugs on lactate metabolism and gluconeogenetic synthesis. The cell growth and metabolism have been characterized in a 33 mM lactate medium, non-supplemented or supplemented by dlmalate or by I-citrulline alone or by the compound formed by the stoichiometric combination of the two components: the citrulline-malate (Stimol"). The malate of the complex accelerated the ammonium disappearance, while the citrulline facilitated the lactate consumption. A synergistic action of the complex, by comparison with the additive effects of the individual components, on most of the parameters studied was detected. A remarkable resistance to anoxia, and a quicker recovery under aeration of the cells supplemented with CM, were evident: after carbonation for 2 min the total nucleotides in the medium were increased by 44 per cent with an unchanged energy charge; and after a prolonged (20 min) anoxia followed by an aeration, the capacities of the cells to synthesize ATP in the presence of excesses of both ADP and phosphate were two-fold higher in Stimol" treated cells than in control.

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