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Use of a Lipophilic Cation for Determination of Membrane Potential in Neuroblastoma-glioma Hybrid Cell Suspensions

✍ Scribed by David Lichtshtein, H. Ronald Kaback and Arthur Joel Blume


Book ID
123655605
Publisher
National Academy of Sciences
Year
1979
Tongue
English
Weight
907 KB
Volume
76
Category
Article
ISSN
0027-8424

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Membrane potential and sodium flux in ne
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## Abstract The Na^+^ uptake into neuroblastoma x glioma hybrid cells was measured in Hepes‐buffered EMEM containing 10% calf serum and 5 mM ouabain in the presence and absence of amiloride (1.0 mM). Amiloride was found to markedly inhibit net Na^+^ influx (by approximately 50%). Examination of the