Use of a genetic algorithm in the development of a synthetic growth medium for Arthrobacter simplex with high hydrocortisone Δ1-dehydrogenase activity
✍ Scribed by D. Weuster-Botz; V. Pramatarova; G. Spassov; C. Wandrey
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1995
- Tongue
- English
- Weight
- 832 KB
- Volume
- 64
- Category
- Article
- ISSN
- 0268-2575
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✦ Synopsis
The production process for Arthrobacter simplex with high specific hydrocortisone A'-dehydrogenase activity was improved by medium optimization in parallel shake flask experiments. Using a genetic algorithm, the concentrations of 12 medium components (mineral salts and amino acid) were optimized within 144 experiments to give maximum specific hydrocortisone A'dehydrogenase activity and biomass yield (full experimental plan: 1.5 x loL4 experiments). The specific hydrocortisone A'-dehydrogenase activity was improved by a factor of 4.5 to 1800 U g-'. With the optimized medium composition in a stirred tank reactor for batch production of A. simplex, a specific hydrocortisone A'-dehydrogenase activity of 2000-3000 U g -' dry biomass was achieved depending on harvest time (previously reported data: 18-37 U g-').