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Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase

✍ Scribed by Nicole Dousset; Anne Negre; Robert Salvayre; Pierre Rogalle; Quoc Quan Dang; Louis Douste-Blazy


Book ID
112773679
Publisher
Springer-Verlag
Year
1988
Tongue
English
Weight
379 KB
Volume
23
Category
Article
ISSN
0024-4201

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Biotinylation of Lipoprotein Lipase and
✍ P. Sivaram; S. Wadhwani; M.G. Klein; A. Sasaki; I.J. Goldberg πŸ“‚ Article πŸ“… 1993 πŸ› Elsevier Science 🌐 English βš– 545 KB

Lipoprotein lipase (LPL) and hepatic triglyceride lipase (HL) were biotinylated using N-hydroxysuccinamide ester of biotin (25-fold molar excess) which was incorporated into the lysine amino groups of the enzyme protein. By assessing enzyme activity and heparin-agarose affinity a biotinylation proto