Use of a Flow Cytometric Assay to Quantitate Apoptosis in Human Lymphocytes
โ Scribed by Thomas W. McCloskey; Naoki Oyaizu; Maria Coronesi; Savita Pahwa
- Book ID
- 115594123
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 342 KB
- Volume
- 71
- Category
- Article
- ISSN
- 1090-2341
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Background: Human peripheral blood lymphocytes kept in culture after isolation die by an apoptotic process. Detection of apoptosis with labeled Annexin V to demonstrate loss of plasma membrane asymmetry is sensitive, specific, and easy using flow cytometry. This is true in lymphoblastic cell lines w
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One of the early events occurring at the cell membrane during apoptosis is the translocation of phosphatidylserine from the inner side of the plasma membrane to the outer layer. These phosphatidylserine groups can be bound by fluorescein isothiocyanate (FITC)-labelled annexin V. The aim of this stud
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