Use of a continuous-flow cell separator in density gradient isolation of lymphocytes
β Scribed by C.S Carter; S.F. Leitman; H. Cullis; L.M. Muul; K. Nason-Burchenal; S A. Rosenberg; H.G. Klein
- Book ID
- 114438478
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 375 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0372-1248
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An instrument and procedure for electrophoresis with continuous optical scanning densitometry, automated data processing, and related methodology are described for the continuous analysis of electrophoresis and unity gravity sedimentation of macromolecules or cells in a static density gradient syste
## Abstract Cytoplasts prepared from L929 or Hepaβ2 cells were separated from whole cells using density gradients of renografin. Using this technique, cytoplasts can be isolated from cell lines which cannot be routinely enucleated with an efficiency of 100%. The purified cytoplasts excluded the vit