✦ LIBER ✦

Use of a computer to assay motility in bacteria

✍ Scribed by Brian M. Sager; Jeff J. Sekelsky; Philip Matsumura; Julius Adler

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 520 KB
Volume: 173
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90189-3

No coin nor oath required. For personal study only.

✦ Synopsis

An assay was developed to study the movement of free-swimming Escherichia coli. Cells were videotaped through a microscope, and the videotape images were then digitized and analyzed with a computer. Angular and linear speeds were measured for wild-type E. coli and for a smooth and a tumbly mutant. The average angular and linear speeds of a population were directly and inversely proportional, respectively, to the time spent tumbling. Changes in angular and linear speeds were followed during the response of wild-type E. coli to attractant or repellent.

📜 SIMILAR VOLUMES

Random motility of swimming bacteria in

Random motility of swimming bacteria in restricted geometries

✍ Sherri A. Biondi; John A. Quinn; Howard Goldfine 📂 Article 📅 1998 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 756 KB

Use of Computer-Aided Screening for Dete

Use of Computer-Aided Screening for Detection of Motility Mutants in Zebrafish Embryos

✍ Charles A. Lessman 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 469 KB

## Use of Computer-Aided Screening for Detection of Motility Mutants in Zebrafish Embryos T he zebrafish (Danio rerio) is an exquisite vertebrate model system for the study of developmental mechanisms. The unraveling of these developmental processes at the molecular level will allow design of new

Rapid determination of bacteria in drink

Rapid determination of bacteria in drinking water using an ATP assay

✍ Rolf A. Deininger; JiYoung Lee 📂 Article 📅 2001 🏛 John Wiley and Sons ⚖ 180 KB

## Abstract The presently used heterotrophic plate count (HPC) for the evaluation of the total number of bacteria in a sample of drinking water takes 7 days of incubation. When the results are known, the water has been consumed and is ineffective for the protection of the health of the consumers. O

Actin-dependent motility of melanosomes

Actin-dependent motility of melanosomes from fish retinal pigment epithelial (RPE) cells investigated using in vitro motility assays

✍ McNeil, E. L. ;Tacelosky, D. ;Basciano, P. ;Biallas, B. ;Williams, R. ;Damiani, 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 690 KB

n-ethylmaleimide and ethacrynic acid inh

n-ethylmaleimide and ethacrynic acid inhibit kinesin binding to microtubules in a motility assay

✍ Walker, R.A. ;O'Brien, E.T. ;Epstein, D.L. ;Sheetz, M.P. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 280 KB

Treatment of proteins in vitro with sulfhydryl (SH)-reactive compounds has been used successfully to determine protein regions critical for normal function. To probe structure-function relationships in the microtubule (MT) motor kinesin, the motor was treated with two SH reactive compounds, n-ethylm

Use of Computer-Assisted Motion Analysis

Use of Computer-Assisted Motion Analysis for Quantitative Measurements of Swimming Behavior in Peritrichously Flagellated Bacteria

✍ Charles D. Amsler 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 104 KB