✦ LIBER ✦

Use of a centromere-specific DNA probe (p82H) in nonisotopic in situ hybridization for classification of micronuclei

✍ Scribed by Pia Becker; Harry Scherthan; Prof. Heinrich Zankl

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 306 KB
Volume: 2
Category: Article
ISSN: 1045-2257
DOI: 10.1002/gcc.2870020111

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The DNA probe p82H was used to visualize centromeric DNA in micronuclei (MN) of human cells. Slides prepared from cultures treated by the aneugen (causing aneuploidy) colcemid showed significantly more MN with centromeric signals than those treated by the clastogen (causing chromosome breakage) bleomycin. These results indicate that in situ hybridization with the alphoid p82H DNA probe is a suitable method with which to distinguish between MN containing whole chromosomes and acentric fragments, and hence allows one to discriminate between the clastogenic and aneugenic effects in MN formation.

📜 SIMILAR VOLUMES

Use of centromeric and telomeric DNA pro

Use of centromeric and telomeric DNA probes in in situ hybridization for differentiation of micronuclei induced by lomefloxacin

✍ Sabry M. Attia 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 153 KB

Centromere detection in vinblastine- and

Centromere detection in vinblastine- and radiation-induced micronuclei of cytokinesis-blocked mouse cells by using in situ hybridization with a mouse gamma (Major) satellite DNA probe

✍ K. Salassidis; R. Huber; H. Zitzelsberger; M. Bauchinger 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 916 KB

Cytogenetic analyses of hepatocellular c

Cytogenetic analyses of hepatocellular carcinoma by in situ hybridization with a chromosome-specific DNA probe

✍ Hiroyuki Kimura; Keizo Kagawa; Takeshi Deguchi; Tomoki Nakajima; Masamichi Kakus 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 595 KB

## BACKGROUND. Numerical chromosome analysis has been established in solid tumors by using in situ hybridization ( 1 9 1 ) with a cliromosome-specific probe. We analy& human hepatocellular carcinoma [HCC) by I S 1 for chrornosonic 17 and investigated the correlation o f its copy number with histol