Use of a celite-potassium tert.-butoxide column for rapid saponification of microgram amounts of glycerides at room temperature

✍ Scribed by Daniel P. Schwartz

• Book ID: 104145851
• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 486 KB
• Volume: 161
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85222-5

✦ Synopsis

In a simple, two-phase procedure, a capillary tube containing a bed of potassium tert.-butoxide supported on Celite 545 is used to saponify microgram amounts of glycerides applied toit in n-hexane. The reaction is carried out at room temperature and is complete wit&h 3 min. The fatty acids are recovered from their soaps by &idification of the column with hydrogen chloride and extraction with hexane or carbon disulfide. They can be recovered in 91-97 ok of theory, if desired, and.analyzed by gas-liquid chromatography. As little as 1 pg of peanut oil was successfully saponified and qualitatively analyzed for its constituent fatty acids.