Surface adsorption of recombinant human
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Serena D. Webb; Stephen L. Golledge; Jeffrey L. Cleland; John F. Carpenter; Theo
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Article
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2002
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John Wiley and Sons
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English
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Recombinant human interferon-gamma (rhIFN-gamma) was lyophilized or spray-lyophilized in 9.5% trehalose, +/- 0.12% polysorbate 20 in 10 mM potassium phosphate, pH 7.5. We measured recovery of soluble protein after spraying, freeze-thawing, and drying and reconstitution. Infrared spectroscopy showed