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Urine as another potential source for template DNA in polymerase chain reaction (PCR)

✍ Scribed by Sachiko Hayakawa; Osamu Takenaka

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 137 KB
Volume: 48
Category: Article
ISSN: 0275-2565
DOI: 10.1002/(sici)1098-2345(1999)48:4<299::aid-ajp5>3.0.co;2-g

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✦ Synopsis

This technical note examines the potential for preparing template DNA in polymerase chain reactions (PCR) from urine in Japanese macaques (Macaca fuscata). Microsatellite band patterns from urine samples showed close agreement with those of blood and fecal samples, and only a few hundred µl of urine yielded a template DNA for PCR. This research will increase the opportunity for scientists to examine the genetic backgrounds of their target animals by using non-invasive sample collection in the wild. Am.

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