Urine analysis of platinum species derived from cis-dichlorodiammineplatinum(II) by high-performance liquid chromatography following derivatization with sodium diethyldithiocarbamate
✍ Scribed by Steve J. Bannister; Larry A. Sternson; A.J. Repta
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 776 KB
- Volume
- 173
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
A clinically useful method is described for the quantitative analysis of platinum species derived from cis-dichlorodiammineplatinum(II) in urine. The drug and its biodegradation products are derivatized directly in urine by reaction with sodium diethyldithiocarbamate (DDTC) to form a common product, a 2:1 DDTC-platinum adduct. This complex is stable and can be quantitatively extracted into 0.1 volumes of chloroform. An aliquot of the chloroform layer is then subjected to high-performance liquid chromatography on a @ondapak CN column and the eluent monitored spectrophotometrically at 2%! nm. At this wavelength the DDTC-platinum adduct has a molar absorptivity of 43,CXlO, and platinum levels of 25 ng/ml of urine can be detected with a precision of & 2.5% and ati accuracy of & 4%.