Uridine and dolichyl diphosphate activated oligosaccharides are intermediates in the biosynthesis of the S-layer glycoprotein ofMethanothermus fervidus
✍ Scribed by Evamarie Hartmann; Helmut König
- Book ID
- 104764126
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 771 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
The surface of the extremely thermophilic archaebacterium Methanothermus fervidus is covered by glycoprotein subunits. The carbohydrate moiety of the surface glycoprotein accounts for about 17 mol%. It is composed of mannose, 3-O-methylglucose, galactose, N-acetylglucosamine and N-acetylgalactosamine. From cell extracts the corresponding surgar-l-phosphates and nucleotide activated derivatives of Man, Gal, GIcNAc and GalNAc were isolated. Furthermore UDP-and dolichyl activated oligosaccharides were obtained. On the basis of the isolated precursors a pathway for the biosynthesis of the oligosaccharide chains is proposed.