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Urease. VI: A new method of assay and the specific enzymic activity

✍ Scribed by George Gorin; Chang-Chen Chin

Publisher
Elsevier Science
Year
1966
Tongue
English
Weight
635 KB
Volume
17
Category
Article
ISSN
0003-2697

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✦ Synopsis

This paper describes a novel method of assaying urease (urea amidohydrolase, EC 3.5.1.5) which affords certain important advantages over other methods. This method utilizes titration with alkali and will therefore be called the "alkalimetric method." Also presented is a definition of the unit of activity in accord with hhe recommendations recently made by the International Union of Biochemistry (IUB) (1, 2). The companion paper that follows (3) reports some observations on the assay method of Sumner (4), which has been widely used in the past'. A factor for interconverting the results obtained by these two assays will be given. The present paper also reports values of the specific act'ivity found recently for highly purified urease preparations and summarizes pertinent earlier result's. MATERIALS

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