Urea sensors based on glass pH electrodes with physically immobilized urease

✍ Scribed by Robert Koncki; Piotr Leszcyński; Adam Hulanicki; Stanislaw Gła̧b

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 572 KB
• Volume: 257
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)80151-v

✦ Synopsis

Dtierent gels were used as a matrix for physlcal enzyme lmmoblhzatlon on the sensmg surface of glass pH electrodes The recommended techmque of lmmobtllzatlon 1s based on enzyme entrapment wlthm a gel prepared m situ on the glass electrode surface Polyivmyl chloride),, cellulose trnntrate and especially cellulose tnacetate are the best matnx materials of ureasc unmobllrzatlon The effect of buffer pH and buffer capacity and the effect of the stlrrmg rate were mvestlgated and the optimum measurement condltlons are discussed Ikywordr Potentlometry, Blosensor, pH, Urea