We examined the route of uptake of 2-aminoethylphosphonate (NEthPo) and of phenylphosphonate (PhePo; 10 mM each) in perfused liver by 31 P-NMR. Uptake of NEthPo was concentrative. The rate of uptake was reduced to 21 AE 2% (n = 3; all percentages refer to control rates) by substituting choline for N
Uptake of marker particles by in vitro ventilated and perfused rat lung
β Scribed by Vidi?, Branislav
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1973
- Tongue
- English
- Weight
- 326 KB
- Volume
- 138
- Category
- Article
- ISSN
- 0002-9106
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β¦ Synopsis
Abstract
Colloidal gold marker was present in the capillary lumen and at various stages of pinocytosis and passage through the endothelial cells. It was occasionally located in the periβendothelial space, close to the basement membrane. The uptake of marker by other cells was not observed. These results suggest the existence of two barriers, the biological (pinocytotic and hydrolytic capacities of the endothelial cells) and mechanical (permeability of the basement membrane).
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Methotrexate (MTX) and cyclosporine (CyA) are coadministered in a number of diseases. In this study, the effects of CyA on the hepatobiliary disposition of MTX were investigated in an isolated perfused rat liver model. A bolus 5-mg dose of MTX was added to a recirculating perfusate in the absence or