Uptake of biotin by human hepatoma cell line, Hep G2: A carrier-mediated process similar to that of normal liver
✍ Scribed by Hamid M. Said; Thomas Y. Ma; Vaijinath S. Kamanna
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 736 KB
- Volume
- 161
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Little is known about the cellular and molecular regulation of the uptake process of the water-soluble vitamin biotin into liver cells, the major site of biotin utilization and metabolism. Such studies are best done using a highly viable and homogeneous cellular system that allows examination of prolonged exposure to an agent($ or a particular condition(s) on the uptake process. Isolated hepatocytes when maintained in primary culture lose their ability to transport biotin by the specialized carrier system. The aim of the present study was, therefore, to examine the mechanism(s) of biotin uptake by the cultured human-derived liver cells,