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Uptake of 2-deoxy-D-glucose by cortical synaptosomes from the long—evans rat

✍ Scribed by D. D. Wheeler; R. G. Hollingsworth

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
623 KB
Volume
4
Category
Article
ISSN
0360-4012

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✦ Synopsis

Uptake of 2-deoxy-D-glucose (2-DC) has been studied in synaptosomes from the Long-Evans rat. Although there are some differences, the results are generally similar t o those reported for Sprague-Ihwley rats. Both D-glucose and 2-DG are taken up by the same carrier, since D-glucose was found t o inhibit uptake of 2-DG. Uptake is highly dependent on a source of metabolic energy, since incubation at O 0 C completely eliminated uptake, while l o r 3 M 2, 4-dinitrophenol (DNP) inhibited uptake by 76.5%. centration and the data fitted t o a model. Sodium is found t o inhibit uptake. A model assuming noncompetitive inhibition by sodium gives best fit t o the data. Thus both Kt and apparent Vm are dependent on the sodium concentration. Although these effects are small, Kt increases with "a], while apparent Vm declines.

Uptake has also been measured as a function of both 2-DG and sodium con-Key words: transport, 2-deoxy-D-glucose, synap tosomes, rat, cortex

Glucose is the primary substrate for energy metabolism in brain tissue [Bachelard and Mcllwain, 19691. Studies utilizing tissue slices have indicated that glucose and its analog, 2-deoxy-D-glucose, are taken up by a common saturable mechanism, presumably facilitated diffusion [Joanny et al, 1969; Bachelard, 19711. The effect of sodium on uptake is unclear. Cooke and Robinson 11971 ] found a 33% inhibition of 3-OMG uptake with a sodium concentration of only 50 mM, while Lund-Andersen et a1 [1976] found no effect of sodium o n 3-OMG uptake. Uptake appears t o take place by a low affinity process, since Kt's have been found t o be in excess of 10K3M [Cooke and Robinson, 19711. However. it is possible that kinetic constants determined by use of tissue slices are in error, because of the complexity of the system [Lund-Andersen and Kjeldsen, 1976; Imd-

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