Uptake and Reduction of Arsenate by Dunaliella sp.

✍ Scribed by Osamu Takimura; Hiroyuki Fuse; Katsuzi Murakami; Kazuo Kamimura; Yukiho Yamaoka

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 346 KB
• Volume: 10
• Category: Article
• ISSN: 0268-2605
• DOI: 10.1002/(sici)1099-0739(199611)10:9<753::aid-aoc573>3.0.co;2-v

✦ Synopsis

Uptake and reduction of arsenate [AS(V)] by Dunaliella sp. cells were determined to investigate the metabolic processes of arsenic in the alga. Cellular uptake of arsenic by Dunaliella sp. cells was markedly affected by the form of arsenic in the medium. The content of arsenic taken up by Dunaliella sp. cells increased rapidly with time on addition of As(V) to the medium. However, in the case of addition of arsenite [As(III)], the gradient of arsenic uptake by Dunaliella sp. cells was low, and arsenic content was small. In the watersoluble fraction of arsenic taken up by Dunaliella sp. cells with exposure to As(V), arsenic was in the forms of organic arsenic, As(V) and As(II1). The content of As(V) in the water-soluble fraction increased with exposure time. The content of As(II1) also increased with time, but remained constant after 5 h of exposure. On the other hand, organic arsenic content was small and did not increase with time. It was found that Duna- lie& sp. takes up As(V) and readily reduces it to As(II1).